Discussion
Yes no problem, let me enlighten you.Puroindolines largely influence cereal grain hardness. In order to understand how they exert this influence, we carried out a molecular analysis of the pina and pinb genes of many Italian wheat cultivars. On the basis of their pin genotypes they could be divided into three groups: Pina-D1a/Pinb-D1a; Pina-D1a/Pinb-D1b; and Pina-D1b/Pinb-D1a. Five cultivars from each group were chosen to be studied to examine the quantity of puroindolines associated with starch (friabilin) and the amount not associated with starch. In addition, the level of pina expression was measured using RT-PCR. Soft cultivars ( Pina-D1a/Pinb-D1a) exhibited the highest level of expression of pina; among the hard cultivars, those with the Pina-D1a/Pinb-D1b genotype showed a lower level of expression, while those with the Pina-D1b/Pinb-D1a genotype did not express pina. Total puroindoline and friabilin content was then measured by flow cytometry. Soft Pina-D1a/Pinb-D1a cultivars displayed high puroindoline content that was primarily starch associated. Hard Pina-D1b/Pinb-D1a cultivars had very low puroindoline content with no puroindoline bound to starch. Hard Pina-D1a/Pinb-D1b cultivars were highly heterogeneous with respect to both the content of puroindolines and the level of association with starch. The accurate quantification of puroindolines in starch-bound and not starch-bound forms in association with molecular analysis, indicates that pina expression and presence controls the abundance of total puroindoline and its association with starch.
Am I or am I not a genius
Am I or am I not a genius
Simpo Two said:
chimera40 said:
By the way, why the hell would you want to know anything about something as dull as this.
That's the problem isn't it - anything that's not pop music or reality TV is now dull and boring. Exit one civilisation, learn to say 'hello' in Chinese.Oh by the way. nín hǎo
Edited by chimera40 on Sunday 20th September 21:37
Well the point behind my post is - grain yield - feed the world etc - it may be 'boring' to Blair's Children who seek instant gratification and 20 A* GCSEs but I'm sure puroindolines are very important to know. It's a great shame that science is so easily sidelined and I think one day we'll regret it - that's all 
Simpo Two said:
Well the point behind my post is - grain yield - feed the world etc - it may be 'boring' to Blair's Children who seek instant gratification and 20 A* GCSEs but I'm sure puroindolines are very important to know. It's a great shame that science is so easily sidelined and I think one day we'll regret it - that's all
OooKkk, and moving quickly on.
)Had a look at google scholar? http://scholar.google.co.uk/scholar?q=Puroindoline...
If you find some articles some of the more scholarlarlalrly members of the PH massive might be able to pull them down from a scientific paper source.
If you find some articles some of the more scholarlarlalrly members of the PH massive might be able to pull them down from a scientific paper source.
Olf said:
Had a look at google scholar? http://scholar.google.co.uk/scholar?q=Puroindoline...
If you find some articles some of the more scholarlarlalrly members of the PH massive might be able to pull them down from a scientific paper source.
Thanks for your reply Olf, but I really don't get what you mean!! If you find some articles some of the more scholarlarlalrly members of the PH massive might be able to pull them down from a scientific paper source.
Would you please expand!!
Cheers
The Moose
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